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Partial purification and characterization of a lymphotoxic factor from multiple sclerosis serum

Department of Medicine, University of California, Irvine, and Orange County Medical Center, Orange, California.

A factor found in the serums of some patients with multiple sclerosis has been shown to have a toxic effect on the normal, mature lymphocyte. This lymphotoxic factor is a protein that is stable to heating at 56°C for 3 hours. It is a nondialyzable material, with recovery of lymphotoxic activity following 30 days of dialysis. Elution of lymphotoxic serum through Sepadex G-100 demonstrates toxic activity closely associated with albumin. Purification by elution through DEAE-cellulose yielded lymphotoxic activity. The lymphotoxic factor was inactivated by standard ammonium sulfate precipitation. Analysis of the effect on distinctive cell types within the total lymphocyte population demonstrated that the T lymphocyte was particularly sensitive.

Received for publication August 1, 1974.

Dr. Armentrout's address is Orange County Medical Center, 101 City Drive South, Orange, CA 92668.