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Optimal detection of Oligoclonal bands inCSF by silver stain

Department of Neurology, Health Sciences Center, SUNY at Stony Brook, Stony Brook, NY.

We applied a sensitive silver nitrate stain to SDS-polyacrylamide gels to detect oligoclonal bands in unconcentrated CSF. Silver staining took less than 3 hours and gave easily readable bands in 93% of MS patients. In contrast, standard Coomassie Blue stain was positive in only 53%. Maximum resolution and numbers of bands resulted when silver staining was used on 10 to 20 microliters of CSF. Silver staining of SDS-polyacrylamide gels permits reliable oligoclonal band detection on small volumes of unconcentrated CSF.

Address correspondence and reprint requests to Dr. Coyle, Department of Neurology, HSC, T-12, SUNY at Stony Brook, Stony Brook. NY 11794.

Supported by the Kroc Foundation and the National Multiple Sclerosis Society (RG1360A2).

Presented in part at the thirty-fifth annual meeting of the American Academy of Neurology, San Diego, CA, April 1983.

Accepted for publication March 11, 1983.