Neurology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Correspondence:
Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when Correspondence are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Shapshak, P.
Right arrow Articles by Hawkins, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shapshak, P.
Right arrow Articles by Hawkins, S.
NEUROLOGY 1985;35:1605
© 1985 American Academy of Neurology

Subacute sclerosing panencephalitis

Measles virus matrix protein nucleic acid sequences detected by in situ hybridization

P. Shapshak, PhD, W. W. Tourtellotte, MD, PhD, S. Nakamura, MD, PhD, M. C. Graves, MD, M. Darvish, MD, D. Hoffman, MD, M. J. Walsh, MD, G. C. Fareed, MD, P. Schmid, MD, C. Heinzmann, BA, K. Sidhu, PhD, E. Bedows, PhD, S. Rozenblatt, PhD, K. Berry, MD and S. Hawkins, MB, MRCP

From the Neurology and Research Services, VA West Los Angeles Medical Center, Wadsworth Division, and Department of Neurology, Reed Neurological Research Center, University of California at Los Angeles School of Medicine (Drs. Shapshak, Tourtellotte, Darvish, Hoffman, Walsh, and Schmid), Los Angeles, CA Division of Clinical Neurology, Institute of Brain Diseases, Tohoku University School of Medicine (Dr. Nakamura), Sendai, Japan; Department of Neurology, Reed Neurological Research Center, University of California at Los Angeles School of Medicine (Drs. Shapshak, Tourtellotte, Graves, Darvish, Walsh, and Schmid), Los Angeles, CA the Molecular Biology Institute, University of California at Los Angeles (Drs. Fareed, Heinzmann, and Sidhu), Los Angeles, CA; Ingene Inc. (Dr. Fareed) Santa Monica, CA; the Departments, of Anesthesiology and Epidemiology, University of Michigan Medical School (Dr. Bedows), Ann Arbor, MI; the National Institutes of Health, Bethesda, MD, and Department of Virology, Weizmann Institute (Dr. Rozenblatt), Rehovot, Israel; the Department of Pathology, Vancouver General Hospital (Dr. Berry), Vancouver, British Columbia, Canada; and the Department of Neurology, Queen's University (Dr. Hawkins), Belfast, UK.

Subacute sclerosing panencephalitis (SSPE) is characterized by a hyperimmune state toward the polypeptides of measles virus except the matrix (M) protein. Using cloned (3H)-labeled complementary DNA probes for in situ hybridization, we found the IM protein and nucleocapsid (NP) protein nucleotide sequences in glial cells and neurons of cryostat sections from two SSPE brains. In one SSPE brain, M protein was lacking, but the other measles polypeptides were present. IgG and IgM antibodies eluted from that brain lacked antibodies to M protein, but antibodies to other measles polypeptides were present. In SSPE brain, the viral M-protein defect is not a deletion of the M gene, but rather a block in gene expression.

Address correspondence and reprint requests to Dr. Shapshak, Neurology Service (W127A), VA Wadsworth Medical Center, Los Angeles, CA 90073.

Supported in part by grants from the Kroc Foundation and Veterans Administration (Merit Review). This project has been funded in part with federal funds from the Department of Health and Human Services under contract no. N01-NS-0-2332.

Accepted for publication February 20, 1985.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1985 by AAN Enterprises, Inc.