NEUROLOGY 1986;36:454-458
© 1986 American Academy of Neurology

ARTICLES

Gangliosides GM1 and GD1b are antigens for IgM M-protein in a patient with motor neuron disease

L Freddo, RK Yu, N Latov, PD Donofrio, AP Hays, HS Greenberg, JW Albers, AG Allessi and D Keren

We studied a patient with an IgM M-protein and lower motor neuron disease to identify the antigens to which the M-protein bound. Gangliosides from peripheral nerve and spinal cord were separated by high-performance thin-layer chromatography and immunostained with the patient's serum. The serum IgM immunostained two gangliosides identified as GM1 and GD1b, and immunostaining was specific for the M- protein light chain type. IgM-binding to the two gangliosides was detectable by ELISA at serum dilutions of greater than 1:10,000, and the M-protein was selectively immunoabsorbed by liposomes containing GM1 or GD1b. The IgM M-protein also bound to asialo-GM1, indicating reactivity to the galactosyl(beta 1-3)N-acetylgalactosaminyl moiety shared by GM1, GD1b, and asialo-GM1.

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