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© 1995 American Academy of Neurology Diversity of T-cell receptor Valpha, Vbeta, and CDR3 expression by myelin basic protein-specific human T-cell clones
From the Departments of Neurology (Dr. Richert and E. Robinson, K. Camphausen, and M. Faerber) and Microbiology and Immunology (Drs. Richert and Hurley), Georgetown University Medical Center, Washington, DC; and the Neuroimmunology Branch (Drs. Martin, Voskuhl, and McFarland), NINDS, National Institutes of Health, Bethesda, MD. We sequenced the cDNAs of alpha and beta T-cell receptors (TCRs), including V, J, and CDR3 regions, expressed by 54 myelin basic protein (MBP)-specific T-cell clones generated from the peripheral blood of 15 multiple sclerosis (MS) patients and three normal controls. Thirteen Valpha gene segments, 18 Vbeta gene segments, 23 CDR3alpha sequences, and 30 CDR3beta sequences were represented among these clones. Some CDR3 motifs were common to several clones that shared epitope specificity, while other motifs were common to clones with diverse epitope specificities. The extensive heterogeneity of TCR gene expression in the human immune response to MBP indicates that therapeutic strategies aimed at blocking a limited number of TCRs are unlikely to fully suppress the T-cell response to MBP in vivo. NEUROLOGY 1995;45: 1919-1922 This article has been cited by other articles:
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