HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Figures Only Full Text Full Text (PDF) Correspondence: Submit a response Alert me when this article is cited Alert me when Correspondence are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Beeson, D. Articles by Vincent, A. Search for Related Content PubMed PubMed Citation Articles by Beeson, D. Articles by Vincent, A.

A transfected human muscle cell line expressing the adult subtype of the human muscle acetylcholine receptor for diagnostic assays in myasthenia gravis

From the Neurosciences Group, Institute of Molecular Medicine, John Radcliffe Hospital, Oxford, United Kingdom.
Supported by the Muscular Dystrophy Group of Great Britain, grant R03/406/1.
Received February 13, 1996. Accepted in final form April 17, 1996.
Address correspondence and reprint requests to Dr. D. Beeson, Neurosciences Group, Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford OX3 9DU, UK.

Immunoprecipitation of human acetylcholine receptor (AChR) is used in the diagnostic assay for myasthenia gravis (MG).We compared human AChR derived from TE671 cells, which express fetal-type AChR, with AChR from TE671-epsilon cells, which we have engineered to express adult-type AChR. Some low-titer MG sera distinguished strikingly between the two subtypes. Four out of seven MG sera that had equivocal titers in standard assays gave positive titers with TE671-epsilon AChR, whereas only one out of seven gave a positive titer with TE671 cells. The new cell line provides a greater concentration of adult AChR than can be obtained from normal human muscle and increases the sensitivity of the diagnostic assay.

NEUROLOGY 1996;47: 1552-1555

This article has been cited by other articles:

				L. Yu, J. Li, S. Ma, J. Jiang, T. Wang, Z. Gamliel, Y. Jing, X. Zhang, and M. J. Krasna Different Characteristics of Nonthymomatous Generalized Myasthenia Gravis With and Without Oropharyngeal Involvement Ann. Thorac. Surg., November 1, 2007; 84(5): 1694 - 1698. [Abstract] [Full Text] [PDF]

				M. I. Leite, M. Jones, P. Strobel, A. Marx, R. Gold, E. Niks, J. J.G.M. Verschuuren, S. Berrih-Aknin, F. Scaravilli, A. Canelhas, et al. Myasthenia Gravis Thymus: Complement Vulnerability of Epithelial and Myoid Cells, Complement Attack on Them, and Correlations with Autoantibody Status Am. J. Pathol., September 1, 2007; 171(3): 893 - 905. [Abstract] [Full Text] [PDF]

				Y Nemoto, S Kuwabara, S Misawa, N Kawaguchi, T Hattori, M Takamori, and A Vincent Patterns and severity of neuromuscular transmission failure in seronegative myasthenia gravis J. Neurol. Neurosurg. Psychiatry, May 1, 2005; 76(5): 714 - 718. [Abstract] [Full Text] [PDF]

				R. Croxen, A. Vincent, J. Newsom-Davis, and D. Beeson Myasthenia gravis in a woman with congenital AChR deficiency due to {epsilon}-subunit mutations Neurology, May 28, 2002; 58(10): 1563 - 1565. [Abstract] [Full Text] [PDF]

				R. Croxen, C. Young, C. Slater, S. Haslam, M. Brydson, A. Vincent, and D. Beeson End-plate {{gamma}}- and {{varepsilon}}-subunit mRNA levels in AChR deficiency syndrome due to {{varepsilon}}-subunit null mutations Brain, July 1, 2001; 124(7): 1362 - 1372. [Abstract] [Full Text] [PDF]

				A. Wakkach, S. Poea, E. Chastre, C. Gespach, F. Lecerf, S. De la Porte, S. Tzartos, A. Coulombe, and S. Berrih-Aknin Establishment of a Human Thymic Myoid Cell Line : Phenotypic and Functional Characteristics Am. J. Pathol., October 1, 1999; 155(4): 1229 - 1240. [Abstract] [Full Text] [PDF]