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Molecular mechanisms of cell death in periventricular leukomalacia

From the Neuropathology Unit (Anatomic Pathology Service) (H.K., M.K.), Brugmann University Hospital, Laboratory of Neurophysiology (P.D.), Brugmann University Hospital, and Pediatrics Service (H.K., G.C.), Queen Fabiola Children’s University Hospital, Free University of Brussels, Belgium; and Child Neurology Department (G.S.), CHU Fleurimont, Université de Sherbrooke, Quebec, Canada. M.K. is currently with the Pediatrics Service, Hôpital Universitaire Farhat-Hached, Sousse, Tunisia.

Address correspondence and reprint requests to Dr Kadhim, Neuropathologie, Service d’Anatomo-Pathologie (ANAPAT), Centre Hospitalier Universitaire Brugmann, Place van Gehuchten 4, 1020 Brussels, Belgium; e-mail: Hazim.Kadhim{at}chu-brugmann.be

Objective: To investigate the cytokine-related molecular cascade leading to neural cell death in periventricular leukomalacia (PVL).

Methods: The authors explored potential tumor necrosis factor (TNF) signaling pathways in human brains with PVL and conducted in situ immunohistochemical investigations to search for possible expression of cytokine receptors in these brains. They also investigated likely links to molecules potentially involved in neurocytotoxicity, particularly pathways involving nitrosative-induced apoptosis.

Results: TNF overexpression was associated with immune reactivity for p75TNFR2 and p55TNFR1 receptors in affected PVL areas. p75TNFR2 labeling was intense on cerebrovascular endothelial cells in PVL areas, whereas no vascular p55TNFR1 immunoreactivity was detected therein. Immune labeling for both receptors was detected on many white matter parenchymal cells. In contrast, there was no immune reactivity for either receptor in tissues taken from non-PVL areas. Additionally, in situ overexpression of inducible nitric oxide synthase was found in PVL brain regions where apoptotic cell death was detected.

Conclusions: Both p75TNFR2 and p55TNFR1 receptors and nitric oxide may be implicated in the pathogenesis of periventricular leukomalacia.

Supported partially by grants from FNRS (Belgium) and "Centre Hospitalier Universitaire Brugmann," Brussels.

Disclosure: The authors report no conflicts of interest.

Received September 8, 2005. Accepted in final form March 27, 2006.

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