Data Supplement

Three tables and two appendices; five Word documents.

Files in this Data Supplement:

• E1 - Table (E)T-1: Oligonucleotide primers, PCR product size, PCR annealing temperatures (TA) and dHPLC column temperatures (TC) used for the analysis of the Cp-gene. Each PCR product of exon 3,4 and 13 was screened twice at different elution temperatures to allow optimized detection of heteroduplices; Word document.
• E2 - Table (E)T-2: Enzymes and conditions of restriction analysis of sequence variations of the Cp gene. (Abbreviations: Mut mutation, wt wild type, h hours); Word document.
• E3 - Table (E)T-3: Primer for pyrosequencing to verify exon 9 and 14 substitutions; Word document.
• E4 - (E)A-1 PCR conditions; Word document.
• E5 - (E)A-2 Immunohistochemistry; Word document.