Acute necrotizing encephalopathy with SARS-CoV-2 RNA confirmed in cerebrospinal fluid
Abstract
Here, we report a case of COVID-19–related acute necrotizing encephalopathy where SARS-CoV-2 RNA was found in CSF 19 days after symptom onset after testing negative twice. Although monocytes and protein levels in CSF were only marginally increased, and our patient never experienced a hyperinflammatory state, her neurologic function deteriorated into coma. MRI of the brain showed pathologic signal symmetrically in central thalami, subinsular regions, medial temporal lobes, and brain stem. Extremely high concentrations of the neuronal injury markers neurofilament light and tau, as well as an astrocytic activation marker, glial fibrillary acidic protein, were measured in CSF. Neuronal rescue proteins and other pathways were elevated in the in-depth proteomics analysis. The patient received IV immunoglobulins and plasma exchange. Her neurologic status improved, and she was extubated 4 weeks after symptom onset. This case report highlights the neurotropism of SARS-CoV-2 in selected patients and emphasizes the importance of repeated lumbar punctures and CSF analyses in patients with suspected COVID-19 and neurologic symptoms.
Case report
In early April 2020, a 55-year-old, previously healthy, woman was admitted to the emergency department at a rural hospital due to fever and myalgia. A CT scan of the thorax showed pulmonary ground-glass opacities and consolidations, and PCR analysis of nasopharyngeal swab specimens confirmed the diagnosis of COVID-19. She had been caring for her husband and 90-year-old mother who both had cough and high fever and also tested positive for SARS-CoV-2 RNA.
After returning home the following day, she became lethargic and had difficulty managing the stairs to her bedroom (day 7 after symptom onset, timeline illustrated in the figure). Later that night, she was found unresponsive in bed. At readmission, her temperature was 37.6°C. She was hemodynamically stable and had no respiratory problems. She was stuporous and had multifocal myoclonus. A CT scan of the brain revealed symmetrical hypodensities in the thalami. A lumbar puncture was performed on day 9 that, in the absence of pleocytosis, did not raise suspicion of CNS infection. PCR tests for herpes simplex virus (HSV) and varicella zoster virus (VZV) were negative. However, signs of blood-brain barrier disruption were noted. On day 11, the patient's neurologic status deteriorated, and she was intubated and transferred to the intensive care unit (ICU). A new CT scan showed low-attenuating areas in the thalami and midbrain, and acute necrotizing encephalitis (ANE) related to her SARS-CoV-2 infection was suspected. Treatment with IV immunoglobulin (IVIG) was initiated in addition to acyclovir.

(A) Timeline from symptom debut showing the neurologic status of the patient and the start and duration of the immunotherapies. The graph additionally illustrates the dynamics of inflammatory markers in plasma and CSF. The first and only positive PCR for SARS-CoV-2 in CSF was detected after 3 weeks indicated by red arrows. IVIG, IV immunoglobulin, PLEX, plasma exchange, GCS, Glasgow Coma Scale. (B) The top and middle rows show images from the first MRI scan at day 12, and the bottom row from the follow-up 1 week later. T2 turbo spin echo (B.a) and FLAIR (B.b) show symmetrically increased signal intensity in subinsular regions (surrounding the claustrum) and thalami (red arrow). The same areas had bright signal in trace images from diffusion-weighted images (b 1,000), indicating cytotoxic edema (B.c). Increased signal was also present on FLAIR images in the brain stem (asterisk, B.h) with suggested involvement of trigeminal nerves (without contrast enhancement, B.d). The olfactory tract had normal appearance (not shown). T1-weighted images show distinct signal decrease on the initial scan (B.e), with partial normalization on follow-up and small delineated malacias in the thalami (B.j). There was faint contrast enhancement initially (B.f), more evident on follow-up (boxes; B.k). Initial FLAIR images showed clear symmetrical involvement of medial temporal lobes, hippocampi, and cerebral peduncles (asterisks, B.g), as well as the pons (B.d, B.h). On follow-up, there was substantial decrease of signal changes in hippocampi and mesencephalon (B.l, B.m). Susceptibility-weighted images showed multiple small foci of presumed petechial hemorrhage in central thalami and subinsular regions (red arrows; B.i, B.n).
On day 12, the patient was transferred to the ICU at a tertiary referral hospital. Her neurologic symptoms had by then worsened, with impaired brain stem reflexes. MRI of the brain showed symmetrical pathologic signal patterns on all sequences, compatible with ANE (figure). In CSF from a second lumbar puncture on day 12, the cell count was normal, IgG concentration slightly increased, and albumin concentration had normalized. No specific autoantibodies could be detected, and once again, PCR for HSV, VZV, and SARS-CoV-2 in CSF was negative.
However, CSF biomarkers for neuronal injury including neurofilament light (NfL) and tau were markedly increased. Glial fibrillary acidic protein (GFAp), a biomarker for astrocytic activation and neuroinflammation, was also highly elevated. In addition, interleukin 6 (IL6) in CSF was somewhat increased. At this time, the systemic inflammatory markers (CRP and WBC) were normal to slightly elevated (figure). Neuronal rescue protein levels were significantly increased in the proteomics analysis of CSF. Other proteins that changed during the disease course included lipid transporters, enzyme cofactors, cell surface receptor ligands, apolipoproteins, and innate immune system complement factors, possibly reflecting the dynamics of the underlying pathology. Proteomics data are available from Dryad, https://doi.org/10.5061/dryad.xwdbrv1bb.
On day 14, a scant improvement was noted with an increased level of consciousness and normalization of brain stem reflexes. Continuous EEG monitoring showed generalized showed slow waves without epileptiform activity. A second MRI revealed partial regression of the signal changes in the brain stem and medial temporal lobes, while contrast enhancements in central thalami and subinsular regions were more pronounced (figure). Venography showed patent veins, and no hypoperfusion was detected in this patient (image available at Dryad, https://doi.org/10.5061/dryad.xwdbrv1bb).
Of interest, in the third CSF sampling, rRT-PCR for SARS-CoV-2 targeting the N gene was positive at a cycle threshold value of 34.29. At this low concentration, the finding was not reproducible using a commercial PCR assay (Abbott RealTime SARS-CoV-2; Abbott Molecular, Wiesbaden, Germany). Biomarkers of neuronal injury, NfL and tau, had further increased, whereas GFAp and IL6 had decreased. CSF protein levels were increased, and oligoclonal bands were now detected in CSF.
ANE carries a poor prognosis, and there is no specific therapy because the etiology is unknown. However, because CSF IgG levels were increased, immunotherapy with plasma exchange (PLEX) was started at day 20. We decided against continued treatment with IVIG based on the reported high thromboembolic incidence in patients with COVID-19. The patient received newly collected replacement donor plasma in the hope that it contained SARS-CoV-2 antibodies.
Gradually, the patient's neurologic condition improved, and on day 32, she could nod, spontaneously, move her legs, and point to a photograph of her children. GFAp had normalized, but NfL and tau remained strongly increased (figure). She was extubated on day 35 and discharged to rehabilitation.
Discussion
This case report demonstrates 2 important aspects of SARS-CoV-2: first, that the virus may have significant neurotropism, and second, that normal CSF cell counts and initial negative SARS-CoV-2 RNA PCR results in CSF do not exclude CNS engagement. This case also underscores the value of repeated CSF testing when CNS COVID-19 is suspected.
The first report of a patient with COVID-19 and ANE was published in March 2020.1 In that case, the presence of virus in CSF was not examined. The case had similar radiologic features as reported here: symmetric thalamic lesions, hemorrhagic components, and involvement of medial temporal lobes and subinsular regions. The latter 2 components are not commonly described features of ANE and could possibly reflect a COVID-19–specific aspect of the pathophysiology. An earlier report by Moriguchi et al.2 described a patient with unilateral involvement of the medial temporal lobe where the virus was detected in the CSF.
ANE is recognized as a distinct entity with a rapid onset of neurologic symptoms, most often secondary to a viral infection such as influenza and herpes viruses. MRI findings include bilateral signal changes and necrosis symmetrically in central thalami and sometimes with hemorrhagic components and/or involvement of the anterior brain stem. Despite its association with viral infection, ANE is not usually considered an inflammatory encephalitis. In fact, the absence of CSF pleocytosis is one of the diagnostic criteria for ANE.3 It has been suggested that an intense surge of proinflammatory cytokines causes focal damage to the blood-brain barrier, with edema and necrosis as secondary effects. This cytokine storm or hyperinflammatory state is described in subgroups of patients with viral infections, including COVID-19. In our patient, however, markers for systemic inflammation were only mildly elevated. Immunotherapy in this case led to clinical improvement. This encouraging result, although congruent with a potentially inhibited or halted inflammatory process, should be interpreted with caution.
Neurotropism of coronaviruses and an association with demyelinating lesions have been demonstrated in both human and animal studies.4 Recently, SARS-CoV-2 was detected in brain tissue at autopsy, and viral infection of the neurons was confirmed.5 A possible route of entry could be the trigeminal and olfactory nerves.6 Infiltration through the olfactory system could explain the increased FLAIR signal in the medial temporal lobe.7 The signal changes in the brainstem and thalamus may represent a central infiltration through the trigeminal system. The bilateral alterations in the claustrum are very unusual; the findings might be a consequence of neuronal retrograde dissemination as the claustrum has a central position in the limbic system.
High levels of IgG in CSF and oligoclonal bands indicate an ongoing inflammatory process, and demyelination and axonal injury were confirmed by extremely high levels of NfL and tau.
The virus was detected only after repeated CSF samplings. The single positive test result in CSF coincides with elevated damage markers and could either be a sign of increased virus load/RNA over time, release of virus from damaged nerve cells, or possibly a false-positive test result. The negative test result with the commercial test kit could either be the low sensitivity of the test kit or simply missed by chance. Further studies are needed to confirm this finding and elucidate the route of entry of SARS-CoV-2 into the CNS and the immunologic response in COVID-19–related ANE. The diagnostic and prognostic values of biomarkers for axonal injury and inflammation, MRI, and treatment options with immunotherapy should be key elements in future studies.
Acknowledgment
The authors thank Niklas Edner at Consultant in Clinical Microbiology at Public Health Agency of Sweden for insightful comments and Ganna Shevchenko for support in the proteomics analysis.
Appendix Authors



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Copyright © 2020 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the American Academy of Neurology. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND), which permits downloading and sharing the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.
Publication History
Received: May 14, 2020
Accepted: June 19, 2020
Published online: June 25, 2020
Published in print: September 8, 2020
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The authors report no disclosures relevant to the manuscript. Go to Neurology.org/N for full disclosures.
Study Funding
Supported by the Swedish Research Council and Open Medicine Foundation. HZ and ER are Wallenberg Academy Fellows.
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Virhammar et. al.1 present a case of acute necrotizing encephalopathy (ANE) in a patient with COVID-19 diagnosed by nasopharyngeal swab PCR1. SARS-CoV-2-PCR testing on two CSF specimens performed on days 9 and 12 after symptom onset were negative, while a third CSF specimen tested positive at a cycle threshold (Ct) of 34.29 at 21 days. While interesting, these results do not indicate that SARS-COV-2 is neurotropic. First, most experts consider Ct counts >40 to represent a negative test and counts >34 may not represent meaningful disease.2 Since PCR testing can amplify contaminants, this result may be a false positive, particularly with negative initial CSF testing. Second, since ANE is typically a post-infectious disease, a biologically plausible causal link with a weakly positive CSF PCR at 21 days is not established. Third, a large series of patients with COVID-19 who had neurologic symptoms found SARS-CoV-2 PCR was negative in CSF in 100% of patients.3 Although one study reportedly detected SARS-CoV-2 in a brain autopsy,4 no immunohistochemistry studies were performed and rough endoplasmic reticulum may be mistaken for virions. Larger brain autopsy series using immunohistochemistry have not detected primary viral CNS invasion.5 Direct neurotropism of SARS-CoV-2 has yet to be definitively established.
Disclosure
The authors report no relevant disclosures. Contact [email protected] for full disclosures.
References
Virhammar et al.1 reported a Covid-19 patient who developed an acute necrotizing encephalopathy (ANE) in whom SARS-CoV-2 RNA was detected in the CSF. The authors remarked that, first, SARS-CoV-2 shows a significant neurotropism, and, second, to consider repetitive CSF samples for PCR assessment.1
Several routes used by the neurotropic virus to reach CNS have been described: transneural and hematogenous pathways.2 Following infection of superior airways, SARS-CoV-2 passes through the epithelium and gains access to the bloodstream, leading to a cytokine storm, which might be an important etiologic mechanism to the ANE.3
The olfactory nerve connects the nasal cavity directly with the CNS and might therefore be a fast and easy shortcut for SARS-CoV-2 into the CNS.2,3 Proposed mechanisms are either infection of olfactory receptor neurons (ORNs) themselves or diffusion through channels formed by olfactory ensheathing cells. Subsequent virus spread through the CNS could occur through multiple mechanisms, including trans‐synaptic transport and microfusion. ORNs’ axons pass through the CSF of the subarachnoid space terminating on mitral and tuft cells in the olfactory bulbs. This might explain the presence of SARS-CoV-2 in the CSF.2,4,5
We agree with the authors1 that that repetitive CSF samples should be considered for PCR assessment in Covid-19 patients.
Disclosure
The authors report no relevant disclosures. Contact [email protected] for full disclosures.
References